Cspg4 cre
WebTo determine whether mutations in Ng2/Cspg4-expressing cells could induce sarcomas, we crossed Ng2/Cspg4-Cre mice or Ng2/Cspg4-CreER mice with Trp53flox/flox mice (Marino et al., 2000) to generate Ng2/Cspg4-Cre-mediated Trp53 conditional knockout mice. In the case of mice expressing the Ng2/ Cspg4-CreER allele, the conditional allele … Web008533 B6;FVB- Ifi208 Tg(Cspg4-cre)1Akik /J These transgenic mice express the Cre recombinase under the control of the mouse chondroitin sulfate proteoglycan 4 ( Cspg4 ) …
Cspg4 cre
Did you know?
WebJun 28, 2024 · CSPG4-directed immunotherapy may be approached in several ways, including: (1) antibody-based fusion proteins for the selective delivery of a pro-apoptotic factors such as tumor necrosis factor-related apoptosis-inducing ligand to agonistic death receptors 4 and 5 on the cell surface; and (2) CSPG4-specific immunotoxins which bind … Web[2], CSPG4-dsRed [6 ], CSPG4-TK [2 ], CSPG4-Cre [7 ], and CSPG4-CreERT2 [8], have been generated to target peri-cytes. None of these markers, however, are pericyte-specic. For example, PDGFRβ, CD13, and RGS5 also mark vascular smooth muscle cells (vSMCs) and broblasts in addition to pericytes, while CSPG4 labels pericytes, vSMCs, and
WebMar 30, 2024 · Two samples are replicates from Cspg4-Cre;tdTomato mice with cell enrichment for tdTomato+ cells (tdT1 and tdT2). Two samples derive from age-matched (9-months-old) WT and TNF-Tg, where the WT serves as control to the TNF-Tg mice with chronic inflammatory-erosive arthritis. The WT and TNF-Tg samples were sorted based … WebOct 10, 2024 · Finally, the Cspg4-Cre/Esr1*:Dsp F/F mice did not show evidence of cardiac dysfunction, which would be expected on deletion of Dsp in cardiac myocytes, as reported previously. 19 Consequently, the data indicate that cardiac arrhythmias and conduction defects in the Cspg4-Cre/Esr1*:Dsp F/F mice are independent of cardiac myocytes and …
WebMay 23, 2024 · Cspg4-Cre lines were genotyped by qPCR according to The Jackson Laboratory protocol. Mouse Procedures. For induction of Cre-mediated recombination in NG2-Cre ERTM mice, 1 mg of tamoxifen (Sigma-Aldrich) was injected twice daily for 5 consecutive days as previously described (Kunisaki et al., 2013. Kunisaki Y. WebAug 19, 2024 · The specificity and pattern of cre activation varied significantly across the three lines, under either the PDGFRβ or NG2 promoter (Pdgfrβ-CreRha, Pdgfrβ-CreCsln, and Cspg4-Cre). In particular, a mouse line with Cre under the NG2 promoter resulted in sparse TdTomato labeling of mural cells, allowing for an unambiguous characterization of ...
WebSPG4 Hereditary Spastic Paraplegia is caused by a gene mutation. Gene mutations are small errors in our DNA that can cause big problems. Patients with SPG4 have a …
WebMar 7, 2024 · Results: Fluorescent imaging of Cspg4-Cre;tdTomato vessels demonstrated dim PLVs and strong reporter gene expression in the adjacent superficial saphenous … can goldfish live in freshwaterWebThe latency of the rotarod test was markedly decreased in Cspg4‐Cre; senp1 f/f mice after cerebral ischemia (Figure 3F, Figure S4, Table S1). There was no significant difference in the Y‐maze test between Cspg4‐Cre; senp1 f/f mice and senp1 f/f mice, indicated no influence on learning and memory ability (Figure 3G,H, Figure S4, Table S1 ... fit cc25 bookingWebA single-cell suspension was prepared by pooling the digested SSV and both adjacent PLVs from the hindlegs of Cspg4-Cre;tdT mice (n = 3), which were sequentially sorted by fluorescence activated ... can goldfish live in sandWebJan 10, 2024 · Overexpression of the chondroitin sulfate proteoglycan 4 (CSPG4) has been associated with the pathology of multiple types of such as melanoma, breast cancer, squamous cell carcinoma, mesothelioma, neuroblastoma, adult and pediatric sarcomas, and some hematological cancers. CSPG4 has been reported to exhibit a role in the growth … fitcburg.govWebJan 24, 2024 · Mutation details: A cre/ERT2 open reading frame and Frt-flanked neomycin cassette replaced exon 1 of the Cspg4 (NG2) locus following homologous recombination … can goldfish live in pondsWebMar 21, 2024 · CSPG4 (Chondroitin Sulfate Proteoglycan 4) is a Protein Coding gene. Diseases associated with CSPG4 include Melanoma and Demyelinating Disease.Among its related pathways are Chondroitin sulfate/dermatan sulfate metabolism and Glycosaminoglycan metabolism.Gene Ontology (GO) annotations related to this gene … can goldfish live in saltwaterWeb007576), Cspg4-DsRed (Tg(Cspg4-DsRed.T1)1Akik, stock number 008241, also called NG2-DsRed or NG2DsRedBAC)22 and constitutive Cspg4-Cre (B6; FVB-Tg(Cspg4-cre)1Akik/J, stock number 008533)22 were purchased from Jackson Laboratory. Pdgfrb-Cre23 mouse strain was a kind gift from Dr. Ralf Adams. Mice were genotyped by PCR. … can goldfish live in frozen water